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Demonstration of light chain mRNA in Hodgkin's disease
Author(s) -
Hell Karen,
Pringle James H.,
Hansmann MartinL.,
Lorenzen Johann,
Colloby Peter,
Lauder Ian,
Fischer Robert
Publication year - 1993
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711710211
Subject(s) - immunoglobulin light chain , chain (unit) , messenger rna , medicine , pathology , chemistry , immunology , physics , gene , biochemistry , astronomy , antibody
The lineage of Hodgkin and Reed‐Sternberg cells is still unclear. Detection of both immunoglobulin light chains in Hodgkin and Reed‐Sternberg cells by immunohistochemistry is a well‐known phenomenon. However, up to now, in situ hybridization techniques have failed to demonstrate light chain messenger(m) RNA in Hodgkin and Reed‐Sternberg cells. In this investigation, we have analysed 26 cases of Hodgkin's disease (nodular lymphocyte predominant Hodgkin's disease, mixed cellularity, and nodular sclerosis type) using digoxigenin‐labelled oligonucleotide probes for K and λ light chains by in situ hybridization. In nearly half of the cases of nodular lymphocyte predominant Hodgkin's disease and in one case of mixed cellularity type, mRNA for only one light chain could be clearly demonstrated in the lymphocytic and histiocytic cells, Hodgkin, and Reed‐Sternberg cells. These results support the idea that at least some cases of Hodgkin's disease are B‐cell neoplasms.