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Transforming growth factor alpha and CD68 immunoreactivity in giant cell tumours of bone: A study on the nature of stromal and giant cells, and their interrelations
Author(s) -
Hasegawa Tadashi,
Hirose Takanori,
Seki Kunihiko,
Sano Toshiaki,
Hizawa Kazuo
Publication year - 1993
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711700314
Subject(s) - stromal cell , giant cell , cd68 , pathology , giant cell tumor of bone , histiocyte , biology , lymph node stromal cell , immunohistochemistry , medicine
To clarify the nature of neoplastic cells, 17 giant cell tumours of bone were studied histologically and immunohisto‐chemically. L1 antigen and S‐100 protein were not detected in the tumour giant cells and stromal cells, although present in non‐neoplastic macrophages. The giant cells in all the lesions, some stromal cells, and osteoclasts in the normal bone showed CD68 and transforming growth factor alpha (TGFα) immunoreactivity. Fibrohistiocytic antigen, factor XIIIa, was expressed in large numbers of stromal cells in all lesions. Some stromal cells expressed α‐smooth muscle actin and osteocalcin. These immunohistochemical results suggested that the stromal cells of giant cell tumours of bone showed histiocytic and occasional myofibroblastic and osteoblastic differentiation. Proliferating cell nuclear antigen was demonstrated in the nuclei of the stromal cells only, indicating that these were the sole proliferating elements. TGFα produced by the giant cells and some stromal cells may play a role as a mediator for the attraction and/or proliferation of the precursor cells, and may suppress the activity of osteoblastic stromal cells, resulting in restricted bone formation in giant cell tumours.