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Modulation of cell surface componts of human breast cancer cells by retinoic acid: Enhanced HLA‐DR Expression
Author(s) -
Gardner F. J. E.,
Walker Rosemary A.
Publication year - 1993
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711700105
Subject(s) - retinoic acid , cell culture , receptor , cell , cell growth , cancer research , biology , receptor expression , endocrinology , retinoic acid receptor beta , cell surface receptor , chemistry , medicine , retinoic acid receptor , microbiology and biotechnology , biochemistry , genetics
The effect of the differentiating agent all‐ trans ‐retinoic acid on the expression of components of the milk fat globule membrane and HLA‐DR by breast cancer cell lines has been examined. Effects on proliferation were also considered, to determine whether any cell surface changes were related to or independent of proliferation effects. No significant differences were observed in the expression of components detected by the milk fat globule membrane antibodies HMFG1 and HMFG2 over 8 days culture with 10 ‐7 ‐10 ‐9 M retinoic acid for the cell lines MCF‐7, T‐47 D, ZR‐75, MDA‐MB‐231, and BT‐20. In contrast, there was enhanced expression of HLA‐DR by two oestrogen receptor‐positive cell lines T‐47 D and ZR‐75 and the oestrogen receptor‐negative line MDA‐MB‐231, with differing sensitivities. These effects were independent of inhibition of proliferation, which was only observed for oestrogen receptor‐positive cell lines and for different durations of exposure. The finding of enhanced HLA‐DR expression after retinoic acid treatment has not previously been reported and is of interest regarding clinical potential for the induction of tumour immunity.