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Metabolic requirements for phorbol ester‐induced cytoskeletal changes in renal epithelium
Author(s) -
Rahilly Mave A.,
Fleming Stewart
Publication year - 1993
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711690409
Subject(s) - cytoskeleton , cycloheximide , epithelium , cytochalasin b , microbiology and biotechnology , cytochalasin d , actin , cytochalasin , chemistry , cell , biology , biochemistry , protein biosynthesis , genetics
Alteration of the cytoskeleton and cell–substratum adhesion are important in the progression of renal carcinoma. We have previously shown that treatment of normal human renal epithelium with phorbol esters mimics the changes seen in renal carcinoma cells. In this study we have demonstrated that the phorbol ester‐induced cytoskeletal reorganization is inhibited in the presence of deoxyglucose but not by cycloheximide. We have also shown that treatment of cells with cytochalasin B results in the formation of immature stress fibres restricted to the cell–substratum contact regions. These results suggest that the actin filaments elongate from the focal contacts and that structural rearrangement caused by phorbol esters is an energy‐dependent phenomenon but is independent of de novo protein synthesis.