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Immunohistochemical staining of ras oncogene product in neoplastic and non‐neoplastic mesothelial tissues: Immunoreactivity for N‐ ras and lack of immunohistochemical staining for Ha‐ ras and K‐ ras
Author(s) -
Ramael Marc,
Deblier Ivo,
Eerdekens Christa,
Lemmens Greet,
Jacobs Werner,
Van Marck Eric
Publication year - 1993
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711690406
Subject(s) - immunohistochemistry , staining , pathology , oncogene , biology , medicine , cancer , cell cycle , genetics
Immunohistochemical staining of 36 malignant mesothcliomas and 45 cases of non‐neoplastsic mesothelium including 20 specimens with signs of hyperplasia were investigated using murine monoclonal antibodies directed against p21 ras protein, Ha‐ ras protein, K‐ ras protein, and N‐ ras protein. All cases of non‐neoplastic mesothelium and the majority of the malignant mesotheliomas (78 per cent) showed cytoplasmic and often submembranous immunoreactivity in more than 50 per cent of the cells with both the pan‐ ras and N‐ ras antibody. No immunoreactivity was observed for Ha‐ and K‐ ras . There was no statistically significant difference with respect to immunoreactivity between neoplastic and non‐neoplastic mesothelium or between the various mesothelioma subtypes.