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Expression of the nuclear membrane protein statin in cycling cells
Author(s) -
Ansari Bijan,
Dover Robin,
Gillmore Caroline P.,
Hall Peter A.
Publication year - 1993
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711690402
Subject(s) - statin , nuclear membrane , mitosis , in vitro , population , nuclear protein , lamin , biology , thymidine , microbiology and biotechnology , chemistry , medicine , biochemistry , nucleus , environmental health , gene , transcription factor
Statin is a 57 kD protein previously reported to be expressed by cells in G 0 . We have studied the detailed distribution of statin immunoreactivity in normal human and rat tissues, and correlated this with investigation of in vitro model systems. By laser confocal microscopy, statin immunoreactivity is localized to the nuclear membrane. In contrast to previous reports, using in vitro model systems we found that statin was also expressed by replicating cells as judged by both co‐localization with [ 3 H]thymidine‐labelled and Ki67‐labelled cells. Furthermore, in a nude mouse xenograft model the number of statin‐labelled cells exceeded the number of quiescent cells as assessed by both fraction of labelled mitosis methods and labelling with [ 3 H]thymidine and Ki67. We conclude that although there is an association between expression of the 57 kD nuclear membrane protein statin and growth arrest, this is not absolute and it is expressed in a sub‐population of cycling cells. The properties of statin closely resemble those of nuclear lamins, members of the intermediate filament family.