Premium
A novel monoclonal antibody (FUN‐1) identifies an activation antigen in cells of the B‐cell lineage and Reed—Sternberg cells
Author(s) -
Nozawa Yoshihiro,
Wachi Eiko,
Tominaga Kunihiko,
Abe Masafumi,
Wakasa Haruki
Publication year - 1993
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711690306
Subject(s) - reed–sternberg cell , b cell , antigen , germinal center , monoclonal antibody , biology , microbiology and biotechnology , antibody , pokeweed mitogen , lymphoma , naive b cell , t cell , immunology , concanavalin a , antigen presenting cell , immune system , in vitro , genetics , hodgkin lymphoma
The characterization of a new monoclonal antibody (MoAb) recognizing a human B‐cell activation antigen, designated FUN‐1, is described in this paper. Immunoprecipitation revealed that FUN‐1 recognizes an antigen with a molecular weight (MW) of 75 kD. FUN‐1 reacts with pokeweed mitogen‐activated B lymphocytes and monocytes of peripheral blood, but not with unstimulated lymphocytes or granulocytes. It also reacts with large lymphoid cells in germinal centres, Epstein–Barr virus‐transformed B cell lines, large B‐cell lymphomas, Ki‐1‐positive anaplastic largecell lymphomas, and Reed–Sternberg cells of Hodgkin's disease, but not with Concanavalin A‐activated T cells, acute lymphoblastic leukaemias, T‐cell lymphomas, or low‐grade B‐cell leukaemias. These findings indicate that FUN‐1 recognizes a previously unreported B‐cell activation antigen. This MoAb appears to be useful for the study of maturation and differentiation in the B‐cell lineage as well as for the immunohistochemical diagnosis of B‐cell lymphomas and Hodgkin's disease.