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Detection of calcitonin and calcitonin gene‐related peptide mRNA in human medullary thyroid carcinoma. A retrospective study
Author(s) -
Denijn Maryléne,
De Weger Roel A.,
Van Unnik Jan A. M.,
Otter Wim Den,
Lips Cees J. M.
Publication year - 1993
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711690109
Subject(s) - calcitonin , in situ hybridization , calcitonin gene related peptide , thyroid , medullary carcinoma , thyroid carcinoma , pathology , messenger rna , biology , medicine , neuropeptide , gene , biochemistry , receptor
In Situ hybridization finds many applications in modern pathology. In many cases, special attention is paid to the processing of the tissues prior to in situ hybridization. In order to investigate the value of RNA in situ hybridization (RISH) in retrospective studies, we performed RISH for calcitonin and calcitonin gene‐related peptide (CGRP)‐I and HomRNA in eight medullary thyroid carcinomas processed in 1981–1983. RISH was successful with radioactive calcitonin and CGRP‐1 probes. With biotinylated probes, only calcitcnin‐specific probes gave adequate results. The concentrations of CGRP mRNA were probably too low to be detected by non‐radioactive RISH. The results of RISH were correlated with the immunohistochemical localization of the polypeptides. The results matched in all cases except one, where hybridization for calcitonin mRNA was found, but no immunoreactive calcitonin polypeptide. We con‐clude that RISH can be successfully used for retrospective analysis, even after long storage of tissue embedded in poraffin.