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Detection of capsid antigen of human papillomavirus (HPV) in benign lesions of female genital tract using anti‐HPV monoclonal antibody
Author(s) -
Iwasaki Takuya,
Sata Tetsutaro,
Sugase Motoyasu,
Sato Yuko,
Kurata Takeshi,
Suzuki Kenji,
Ohmoto Hiroshi,
Iwamoto Seiichi,
Matsukura Toshihiko
Publication year - 1992
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711680309
Subject(s) - antigen , capsid , koilocyte , monoclonal antibody , virology , pathology , biology , antibody , dysplasia , condyloma acuminatum , virus , human papillomavirus , medicine , immunology , cervical intraepithelial neoplasia , cervical cancer , cancer , genetics
We established a murine monoclonal antibody (K1H8) to human papillomavirus (HPV) using alkaline‐disrupted virions of HPV type 1 (HPV‐l) as the immunogen. K1H8 recognized a 57 kD capsid protein of HPV‐1 and detected the antigen in paraffin sections of formalin‐fixed tissue. With K1H8, we examined immunchistochemically 68 biopsy specimens obtained from the female genital tract. The specimens were histologically condyloma acuminatum or koilocytotic lesions with or without dysplasia and each specimen was found to harbour a single type of genital HPV, such as types 6, 11, 16, 18, 31, 33, 42, 51, 52, 56, and 58, by Southern blot hybridization analysis. The antigen was localized in the nuclei and occasionally in the cytoplasm of squamous cells showing koilocytotic changes. Eighty‐four per cent of the specimens (57 cases) showed positivity for the antigen, indicating that K1H8 is a broadly‐reactive antibody to various genital HPVs. The results suggest that benign mucosal lesions of the female genital tract are more frequently associated with viral production and are a potential source of transmission.