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Production and characterization of a polyclonal antibody to the c‐erb B‐3 protein: Examination of c ‐erb B‐3 protein expression in adenocarcinomas
Author(s) -
Poller D. N.,
Spendlove I.,
Baker C.,
Church R.,
Ellis I. O.,
Plowman G. D.,
Mayer R. J.
Publication year - 1992
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711680306
Subject(s) - polyclonal antibodies , immunohistochemistry , antibody , microbiology and biotechnology , western blot , biology , adenocarcinoma , pathology , cancer , gene , medicine , immunology , biochemistry , genetics
A polyclonal rabbit antibody was raised to the c‐ erb B‐3 protein using a synthetic peptide corresponding to amino acids 1229–1241 of the predicted protein sequence of c‐ erb B‐3. In Western blot analysis this antibody detects a single band at ˜165kD in a c‐ erb B‐3 transfected (293/HER‐3) human cell line. c‐ erb B‐3 protein expression was then examined in a variety of adenocarcinomas. Expression of c‐ erb B‐3 protein was indicated by membrane and/or cyto‐plasmic tumour cell immunoreactivity in formalin‐fixed, paraffin‐embedded tissue sections. c‐ erb B‐3 protein was detected in a series of 13 out of 14 primary breast carcinomas, 3 of 5 gastric adenocarcinomas, 8 of 9 colonic adenocarcinomas, 2 of 9 prostatic adenocarcinomas, 0 of 6 renal cell carcinomas, 1 of 4 primary lung adenocarcinomas, and 5 of 7 endometrial adenocarcinomas. Immunohistochemical expression of the c‐ erb B‐3 protein appears to be a relatively common event in adenocarcinomas, and further studies are now warranted to establish the role of the c‐ erb B‐3 protein in neoplasia.