Premium
Proliferating cell nuclear antigen (PCNA) expression in Hodgkin's disease
Author(s) -
Schmid Christine,
Sweeney Edel,
Isaacson Peter G.
Publication year - 1992
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711680102
Subject(s) - proliferating cell nuclear antigen , nodular sclerosis , antigen , population , immunophenotyping , cd20 , reed–sternberg cell , lymphocyte , pathology , biology , antibody , microbiology and biotechnology , lymphoma , immunohistochemistry , immunology , medicine , hodgkin lymphoma , environmental health
Previous studies of the proliferating cell fraction in Hodgkin's disease (HD) have been directed towards the classical Hodgkin and Reed–Sternberg cells (HRS) to the exclusion of the background population and have not included cases of nodular lymphocyte predominant Hodgkin's disease (NLPHD). Using an antibody to proliferating cell nuclear antigen (PCNA), we have determined the growth fraction of HRS cells and L&H cells in paraffin sections of 15 cases of classical HD [12 nodular sclerosis (NS), 3 mixed cellularity (MC)] and eight cases of NLPHD. By double staining with anti‐PCNA and antibodies to B cells (CD20) and T cells (CD45RO), we also determined the growth fraction and immunophenotype of the background population in each case. In classical HD, 50·4 per cent of HRS cells were PCNA‐positive and judged to be proliferating, which is comparable to previous studies, while in NLPHD 76·9 per cent of L&H cells were PCNA‐positive. In both classical HD and NLPHD, the majority of PCNA‐positive cells in the background were T cells, which showed a growth fraction of 57·8 and 68·5 per cent, respectively; in comparison, only 4 per cent of B cells were PCNA‐positive in each type of HD. L&H cells are widely accepted to be B cells and there is growing evidence that HRS cells are also B cell‐derived. Our results underline a relationship between classical HD and NLPHD and suggest that the characteristic histological features of both diseases may be caused by the production and release of cytokines from altered B cells.