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The demonstration of a subset of carcinoid tumours of the appendix by in situ hybridization using synthetic probes to proglucagon mRNA
Author(s) -
Shaw Paul A. V.,
Pringle James H.
Publication year - 1992
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711670405
Subject(s) - appendix , proglucagon , biology , in situ hybridization , immunohistochemistry , pathology , haematoxylin , messenger rna , endocrinology , medicine , gene , biochemistry , paleontology , glucagon like peptide 1 , type 2 diabetes , diabetes mellitus
Previous studies using immunohistochemistry have shown variable hormone production by carcinoid tumours of the appendix. In order to confirm the existence of a specific subset of these tumours, in situ hybridization using synthetic oligonucleotide probes to detect prc‐proglucagon and pre‐proinsulin mRNA was performed in formalin‐fixed, paraffin‐ embedded material from eight tubular carcinoids, 12 insulin carcinoids, and two mucinous carcinoids. The results were correlated with standard silver and mucin stains. All tubular carcinoids but none of the insular or mucinouscarcinoids contained proglucagon mRNA. Proinsulin mRNA was not detected in any of the tumours. Tubular carcinoids of the appendix constitute a definable subset of appendiceal carcinoids which have a similar distribution and prognosis to typical insular carcinoids and can be diagnosed on hamaloxylin and eosin‐stained sections confirmed by routine special stains. The main need for recognition is to avoid confusion with mucinous carcinoids. which have a worse prognosis and may require more aggressive treatment.