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HPV in full thickness cervical biopsies: High prevalence in CIN 2 and CIN 3 detected by a sensitive PCR method
Author(s) -
Arends Mark J.,
Donaldson Yvonne K.,
Duvall Edward,
Wyllie Andrew H.,
Bird Colin C.
Publication year - 1991
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711650405
Subject(s) - cervical intraepithelial neoplasia , polymerase chain reaction , primer (cosmetics) , human papillomavirus , dna , papillomaviridae , biology , microbiology and biotechnology , pathology , medicine , gene , chemistry , cervical cancer , cancer , genetics , organic chemistry
A new type‐specific, sensitive, non‐radioactive assay is described for the detection of human papillomavirus (HPV) DNA in tissues. Sequences within the E6 gene were amplified by the polymerase chain reaction (PCR), using primer pairs which clearly distinguish HPV types, including those with close sequency homology such as 6b and 11. The amplified DNA products were identified by non‐radioactive oligonucleotide hybridization and restriction endo‐nuclease mapping, and the method was sufficiently sensitive to detect between 3 and 5 SiHa cells (each containing 1–2) copies of (HPV 16 DNA) amongst 10 000 non‐HPV‐ containing cells. Frozen and archival paraffin sections were equally acceptable substrates for the reaction. The assay was applied to frozen sections full thickness cervical epithelium from 60 cases of cervical intraepithelial neoplasia (CIN) and 24 normal cervical controls. Prevalence of HPV 16 was similar (approximately 50 per cent) in both CIN 2 and CIN 3, and in the whole series HPV 16 was almost five‐fold more common than HPV 18. Low‐risk HPV types were present in 5 per cent of CIN 1, but 0 per cent of CIN2 and CIN3 biopsies. The data emphasize the biological similarity of CIN 2 and CIN 3 lesions, and their divergence from CIN 1.