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New monoclonal antibodies reacting with bile ducts: Further insights into the pathogenesis of bile ductular proliferation in biliary diseases
Author(s) -
Ayres Reuben C. S.,
Hübscher Stefan G.,
Shaw Jean,
Garner Chris,
Joplin Ruth,
Williams Anne,
Neuberger James M.
Publication year - 1991
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711650211
Subject(s) - monoclonal antibody , intrahepatic bile ducts , pathology , immunohistochemistry , biology , stain , staining , antibody , fetus , pathogenesis , monoclonal , bile duct , immunology , medicine , pregnancy , genetics
We have produced a range of monoclonal antibodies which stain human intrahepatic bile ducts of different sizes. Amongst 26 monoclonal antibodies produced, five clones reacted specifically with bile ducts of different sizes, of which three have been maintained in culture and their viability following freezing and thawing confirmed. Staining patterns varied between normal adult liver tissue, normal fetal liver tissue and a variety of hepatobiliary diseases. The antibodies provide further evidence of the immunological heterogeneity of the human intrahepatic biliary tree and support the hypothesis that proliferating bile ductules are derived from periseptal hepatocytes. The preparation of the antiboides, their staining reactions in normal adult, normal fetal and a variety of liver diseases are described.