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Immunohistochemical detection of the epidermal growth factor receptor in paraffin‐embedded human tissues
Author(s) -
Gullick William J.,
Hughes Christine M.,
Mellon Killian,
Neal David E.,
Lemoine Nicholas R.
Publication year - 1991
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711640403
Subject(s) - immunohistochemistry , receptor , epidermal growth factor receptor , epidermal growth factor , antibody , staining , tgf alpha , biology , pathology , microbiology and biotechnology , cancer research , medicine , immunology , biochemistry
Expression of the epidermal growth factor (EGF) receptor was evaluated by immunohistochemical staining of formalin‐fixed, paraffin‐embedded tumour tissues employing two antibodies raised to short synthetic peptides from the cytoplasmic domain of the molecule. Both antibodies gave concordant staining of a series of bladder cancers known to express or lack EGF receptors. There was no cross‐reaction with the related c‐ erb B ‐2 protein, which was also over‐expressed in some cases. Cancers with EGF receptor expression also expressed high levels of TGF‐alpha, a receptor agonist.