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Histopathological studies on the local reactions induced by complete fReund's adjuvant (CFA), bacterial lipopolysaccharide (LPS), and synthetic lipopeptide (P 3 C) conjugates
Author(s) -
Wiedemann Frank,
Link Ralf,
Pumpe Konrad,
Jacobshagen Ulrich,
Schaefer Hans E.,
Wiesmüller KarlHeinz,
Hummel RolfPeter,
Jung Günther,
Bessler Wolfgang,
Böltz Traudel
Publication year - 1991
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711640313
Subject(s) - lipopeptide , lipopolysaccharide , adjuvant , lipid a , chemistry , conjugate , inflammation , immunology , microbiology and biotechnology , bacteria , medicine , biology , mathematical analysis , genetics , mathematics
Abstract The inflammatory reactions following subcutaneous application of adjuvants revealed characteristic pathological patterns. The injection of complete Freund's adjuvant (CFA) resulted in the formation of large lipid deposits encircled by an inflammatory reaction and concentrically arranged collagen bundles. Bacterial lipopolysaccharide (LPS) caused granulomatous aggregations of mononuclear cells with thrombotic vessel occlusions. Inoculation of the lipopeptide adjuvants induced accumulation of mononculear cells with only minimal fibrotic changes which were resolved after day 28. Lipopeptide conjugates based on the head group tripalmitoyl‐ S ‐glyceryl‐cysteinyl‐serin (P 3 CS) can thus be used as effective immunogens and adjuvants without long‐term tissue damage.