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Immunohistological analysis of the immunoreactivity of normal lymphoid cells and lymphomas with the monoclonal antibody OPD4
Author(s) -
O'Grady Jane,
Blaikie Andrew J.,
Howie Sarah E.,
Salter Donald M.,
Krajewski Andrew S.
Publication year - 1991
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711640205
Subject(s) - monoclonal antibody , pathology , antibody , monoclonal , immunohistochemistry , lymphatic system , immunology , biology , medicine
OPD4 is a recently described monoclonal antibody that recognizes a fixation‐resistant 200 kD antigen restricted to a subset of T cells. Immunolabelling with OPD4 in paraffin sections of normal lymphoid tissues and cases of malignant lymphoma was compared with that of other antibodies in common use, including the T‐cell restricted antibodies MTI and UCHL1 and the B‐cell restricted antibodies MB1, F8‐11‐13, and L26. OPD4 showed similar immunoreactivity to UCHL1 in normal tissues. OPD4 did not stain Reed‐Sternberg cells in Hodgkin's disease. In non‐Hodgkin's lymphomas, OPD4, like UCHL1, reacted with only 2/22 B‐cell lymphomas. OPD4 was, however, less useful as a marker of T‐cell lymphomas, staining only 11/32 cases, while UCHL1 stained 22/32 cases. We conclude that OPD4 is not a useful antibody for the routine diagnosis of T‐cell lymphoma.