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Detection of mRNA for ribosomal phosphoprotein P2 in normal colon and colonic tumours by In situ hybridization
Author(s) -
Harvie R.,
Elvin P.,
McArdle C.,
Morten J. E. N.,
McNicol A. M.
Publication year - 1991
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711640112
Subject(s) - in situ hybridization , biology , digoxigenin , phosphoprotein , northern blot , messenger rna , pathology , microbiology and biotechnology , in situ , chemistry , medicine , gene , biochemistry , organic chemistry
We have localized the mRNA for the ribosomal phosphoprotein P2, a putative metastasis‐related sequence, in normal colon and colonic carcinomas by in situ hybridization, using an oligonucleotide probe end‐labelled with digoxigenin. The mRNA was identified in normal colonic epithelial cells, the intensity of the signal being greater in cells at the base of the crypts compared with those on the surface. A strong positive signal was also seen in plasma cells, in fibroblasts in granulation tissue, in ganglion cells, and in hepatocytes. A positive signal was identified in all 16 primary colonic tumours studied and in 7 hepatic metastases In contrast to previous studies based on Northern blot analysis, we were unable to demonstrate increased expression in metastases as compared with primary tumours, nor could we demonstrate any increased expression in primary tumours which were associated with distant metastases.