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A new monoclonal antibody, PM‐2K, specifically recognizes tissue macrophages but not blood monocytes
Author(s) -
Takeya Motohiro,
Tsuchiya Tatsuaki,
Shimokawa Yasuhiro,
Takahashi Kiyoshi
Publication year - 1991
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711630408
Subject(s) - follicular dendritic cells , macrophage , spleen , red pulp , pathology , lymph node , monoclonal antibody , immunoelectron microscopy , antigen , biology , lymph , adipose tissue macrophages , tonsil , antibody , germinal center , immunology , chemistry , antigen presenting cell , immune system , medicine , b cell , t cell , insulin resistance , biochemistry , in vitro , obesity
A new monoclonal antibody, PM‐2K, was raised against 24‐h cultured human peritoneal macrophages. Immunohistochemically, PM‐2K recognized most tissue macrophages in lymphoreticular organs such as the thymus, spleen, lymph node, and tonsil. Kupffer cells of the liver, alveolar macrophages, and macrophages in the interstitial tissue of the kidney, pancreas, and many other organs were also positively labelled. On the other hand, PM‐2K failed to recognize blood monocytes, freshly isolated peritoneal macrophages, microglial cells, osteoclasts, and dendritic cells such as Langerhans cells, interdigitating cells, and follicular dendritic cells. In various pathological conditions. PM‐2K labelled a wide variety of proliferating macrophages. Reaction products of PM‐2K were observed by immunoelectron microscopy on the cytoplasmic membrane of cultured peritoneal macrophages. The molecular weight of the antigen recognized by PM‐2K was determined to be 150 kD by Western blotting. As no cells other than macrophages were reactive with PM‐2K, this antibody is considered to be very useful not only in the investigation of macrophage differentiation and maturation, but also in the diagnosis of various proliferative disorders of macrophages.