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Monoclonal antibodies to cultured human glomerular mesangial cells. I. Reactivity with haematopoietic cells and normal kidney sections
Author(s) -
Stewart Keith N.,
RoyChaudhury Prabir,
Lumsden Lynne,
Jones Michael C.,
Brown Paul A. J.,
Macleod Alison M.,
Haites Neva E.,
Simpson John G.,
Power David A.
Publication year - 1991
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711630313
Subject(s) - monoclonal antibody , mesangium , mesangial cell , antibody , haematopoiesis , antigen , microbiology and biotechnology , renal glomerulus , biology , flow cytometry , alkaline phosphatase , cell culture , kidney , glomerulonephritis , stem cell , immunology , biochemistry , endocrinology , enzyme , genetics
The aim of this study was to produce monoclonal antibodies to cultured human glomerular mesangial cells in order to obtain specific markers for these cells and to aid the study of their function. Using standard monoclonal antibody techniques, 29 hybridomas producing antibodies directed to cultured mesangial cells were obtained. Most of these antibodies were not reactive with normal or neoplastic haematopoietic cell lines by flow cytometry. Fourteen of the 29 culture supernatants bound to various components of normal human kidney sections stained by the alkaline phosphatase/anti‐alkaline phosphatase (APAAP) method. Ten of these supernatants reacted with components within the glomerulus, with six binding to the mesangium. These studies suggest that (1) mesangial cells in culture may show significant de‐differentiation, because most supernatants which reacted with mesangial cells in culture did not do so in tissue sections: (2) antibodies reactive with haematopoietic cells may not detect the majority of immunogenic surface antigens on cells in tissues; and (3) some of the antibodies which we have produced may prove to be useful markers for mesangial cells in glomerular disease.