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Antipeptide antibodies against the pNR‐2 oestrogen‐regulated protein of human breast cancer cells and detection of pNR‐2 expression in normal tissues by immunohistochemistry
Author(s) -
Piggott Nigel H.,
Henry James A.,
May Felicity E. B.,
Westley Bruce R.
Publication year - 1991
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711630204
Subject(s) - immunohistochemistry , biology , complementary dna , antibody , breast cancer , microbiology and biotechnology , messenger rna , antiserum , in situ hybridization , pathology , cancer , gene , medicine , immunology , biochemistry , genetics
Five peptides, corresponding to regions of the predicted protein sequence of the oestrogen‐regulated pNR‐2 protein which is expressed in oestrogen‐responsive human breast cancer cells, were synthesized. Two peptides were immunogenic in rabbits and antisera against one peptide reacted with the pNR‐2 protein in sections of formalin‐fixed, paraffin‐embedded breast tumour. There was a significant correlation between the extent of pNR‐2 protein expression detected by immunohistochemistry and pNR‐2 mRNA levels determined by hybridization with a cDNA probe in a series of primary breast tumours. pNR‐2 expression was assessed immunohistochemically in a panel of normal tissues. Expression was detected in normal breast, small intestine, and stomach (body and antrum).