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Demonstration of Epstein‐Barr viral DNA in formalin‐fixed, paraffin‐embedded samples of Hodgkin's disease
Author(s) -
Libetta Carole M.,
Pringle J. H.,
Angel Carole A.,
Craft A. W.,
Malcolm A. J.,
Lauder I.
Publication year - 1990
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711610313
Subject(s) - lymphoma , dna , pathology , biology , virus , southern blot , microbiology and biotechnology , virology , medicine , genetics
The presence of Epstein‐Barr virus (EBV) DNA in formalin‐fixed, paraffin‐embedded samples of Hodgkin's disease (HD) was investigated by Southern blot hybridization using a specific EBV Bam H1W fragment probe. DNA from 16 samples of HD in children and young adults was compared with that from ten samples in adults older than 45 years. A further eight cases of DNA extracted from fresh samples of HD were also studied, in order to compare the results obtained with fresh and fixed tissue samples. Hybridization was demonstrated in 15 of the 34 cases of HD studied in contrast to 3 or the 34 control specimens of non‐Hodgkin's lymphoma, non‐neoplastic lymph nodes, and carcinomas. No differences between the two age groups compared were found. The results of this study suggest that there is a definite association between EBV and HD, although the exact nature of this association remains to be established. It has also been shown that DNA of sufficient quality for the detection of EBV DNA can be extracted from formalin‐fixed, paraffin‐embedded material, and that comparable results can be obtained using DNA extracted from fresh and fixed tissue.