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Induction of antigen expression of follicular dendritic cells in a monoblastic cell line. A contribution to its cellular origin
Author(s) -
Fliedner A.,
Parwaresch M. R.,
Feller A. C.
Publication year - 1990
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711610112
Subject(s) - microbiology and biotechnology , antigen , follicular phase , cell culture , biology , follicular dendritic cells , immunology , antigen presenting cell , t cell , genetics , immune system
Experiments were carried out to elucidate the cellular origin of the dendritic reticulum cell (DRC). The monoblastic cell line THP‐1, the histiocytic cell line U‐937, and the mononuclear cell fraction from peripheral blood (PMC) were stimulated with supernatants from lectin‐stimulated peripheral blood lymphocytes and from stimulated T‐ and B‐cell lines. Differentiation towards DRC was assessed by immunocytochemical demonstration of the DRC‐specific antigen Ki‐M4. Supernatants from isolated peripheral T lymphocytes and from T‐ and B‐cell lines were capable of stimulating THP‐1 to Ki‐M4 antigen expression, whereas U‐937 and the PMC fraction remained negative for this antigen throughout the experiments. These results provide further evidence for a relationship of DRCs with the mononuclear phagocytic cell system and hence for their bone marrow origin. Furthermore, the data suggest that soluble factors of T and/or B cells are involved in mediating the differentiation process of precursor cells to DRC.