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Immunohistochemical demonstration of altered intracellular localization of the c‐ Myc oncogene product in human colorectal neoplasms
Author(s) -
Williams Alistair R. W.,
Piris Juan,
Wyllie Andrew H.
Publication year - 1990
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711600404
Subject(s) - immunohistochemistry , staining , pathology , monoclonal antibody , oncogene , biology , cytoplasm , carcinoma , positive staining , cell nucleus , antibody , cell , medicine , immunology , cell cycle , microbiology and biotechnology , biochemistry
The distribution of the c‐ myc oncogene product p62 was examined by immunohistochemistry using the monoclonal antibody Mycl‐9E10 in a series of 50 colorectal resections for carcinoma. The specimens were specially handled to ensure rapid fixation in formalin, and a significant improvement was shown in the quality and localization of staining compared with routinely handled specimens. Non‐neoplastic mucosa showed the presence of nuclear staining of epithelial ceiis in 93 per cent of the samples, whilst all carcinomas showed cytoplasmic staining and infrequent nuclear staining. Adenomas showed an intermediate pattern, with significantly more frequent cytoplasmic distribution than non‐neoplastic mucosa, but less than carcinomas. The results show that whilst fixation conditions are important in the immunolocalization of the C‐ myc protein product, there may be a consistent difference between non‐neoplastic mucosa and carcinoma in the manner of association of p62 with the nucleus.