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Microdissection: A novel method for the study of intracellular inclusion bodies
Author(s) -
Martin J. E.,
Swash M.,
Mather K.,
Garofalo O.,
Dale G. E.,
Leigh P. N.,
Anderton B. H.
Publication year - 1990
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711600115
Subject(s) - microdissection , intracellular , extracellular , microbiology and biotechnology , isolation (microbiology) , inclusion bodies , ultrastructure , chemistry , biology , biophysics , pathology , biochemistry , anatomy , medicine , bioinformatics , gene , escherichia coli
The purification of many intracellular and extracellular inclusions is often difficult to achieve due to the low concentration of the abnormalities in the tissue under study, or due to the degradation of components during extraction. We describe the use of microdissection for the isolation of neurons and intraneuronal inclusion bodies. The resulting suspension may be used for biochemical, immunological or ultraslructural studies. The technique is applicable to the study of a wide range of cellular abnormalities.