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Polarized incident light microscopical enhancement of immunogold and immunogold–silver preparations: Its role in immunohistology
Author(s) -
Ellis Ian O.,
Bell Jane,
Bancroft John D.
Publication year - 1989
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711590106
Subject(s) - immunogold labelling , staining , colloidal gold , stain , pathology , biology , optics , ultrastructure , materials science , anatomy , physics , medicine , nanotechnology , nanoparticle
Polarized incident light microscopy (PILM) is a recognized method of visualization of bound colloidal gold in cytological immunocytochemical preparations. This study investigates its role in the assessment of histological sections using both indirect immunogold and immunogold–silver staining methods. With dark field or bright field illumination this technique was found to be advantageous and allowed easy detection staining at low magnification and accurate detection of low levels of stain product. We advocate this technique as a valuable microscopical enhancement method for immunogold immunohistology.