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A comparison of antibodies to type VII and type IV collagen laminin and amnion as epidermal basement membrane markers
Author(s) -
Kirkham N.,
Gibson B.,
Leigh I. M.,
Price M. L.
Publication year - 1989
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711590104
Subject(s) - laminin , amnion , basement membrane , type iv collagen , antibody , type (biology) , chemistry , pathology , biology , immunology , medicine , biochemistry , extracellular matrix , pregnancy , genetics , fetus , ecology
We have compared four monoclonal antibodies which label basement membrane components using an indirect immunoperoxidase method in frozen sections of skin biopsies. The antibodies LH 7·2 and GB3 showed expression limited to epidermal and adnexal basement membrane. Antibodies to laminin and type IV collagen also decorated dermal blood vessels and stromal components. The antibodies LH 7·2 and GB3 are more suitable for labelling epidermal basement membrane in the study of cutaneous lesions.