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Detection of latent virus mRNA in tissues using the polymerase chain reaction
Author(s) -
Lynas Caroline,
Cook Stuart D.,
Laycock Keith A.,
Bradfield John W. B.,
Maitland Norman J.
Publication year - 1989
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711570404
Subject(s) - polymerase chain reaction , virus , herpes simplex virus , reverse transcription polymerase chain reaction , biology , microbiology and biotechnology , reverse transcriptase , virology , gene expression , gene , messenger rna , real time polymerase chain reaction , rna , genetics
The polymerase chain reaction (PCR) has been adapted for use in gene expression studies. Using this technique, we have been able to specifically detect Herpes simplex virus gene expression in the amount of RNA equivalent to that present in a single mouse ganglion. We have also detected specific transcription of the ras oncogene in biopsies of hepatocellular carcinoma tissue. The single tube RNA PCR reaction should be readily adaptable for use as a rapid screening tool in virus diagnosis; it is capable of detecting several virus infections simultaneously using extremely small tissue biopsies.