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Induction of soft tissue tumours in F344 rats by subcutaneous, intramuscular, intra‐articular, and retroperitoneal injection of nickel sulphide (Ni 3 S 2 )
Author(s) -
Shibata Masashi,
Izumi Keisuke,
Sano Nobuya,
Akagi Akiko,
Otsuka Hisashi
Publication year - 1989
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711570314
Subject(s) - desmin , subcutaneous injection , soft tissue , rhabdomyosarcoma , subcutaneous tissue , intramuscular injection , sarcoma , medicine , injection site , cytokeratin , intraperitoneal injection , vimentin , pathology , immunohistochemistry
The carcinogenicity of nickel sulphide (Ni 3 S 2 ) injected into subcutaneous (s.c.), intramuscular (i.m.), or retroperitoneal intrafat (i.f.) tissue, or the intra‐articular space (i.a.) of male F344 rats was studied. Rats were given a single injection of 0.5 mg of Ni 3 S 2 and were observed for 48 weeks. Malignant soft tissue tumours were induced in 18/19 rats (95 per cent) by s.c. injection, 19/20 rats (95 per cent) by i.m. injection, in 16/19 rats (84 per cent) by i.a. injection, and in 9/20 rats (45 per cent) by i.f. injection of Ni 3 S 2 . The i.f. injection of Ni 3 S 2 resulted in a lower tumour incidence and the appearance of tumours 10 weeks later than its injection by other routes. The tumours were examined histologically, ultrastructurally, and immunohistochemically with antibodies against desmin, vimentin, and cytokeratin. The 62 tumours induced by injection of Ni 3 S 2 by different routes were identified as rhabdomyosarcomas (RMS, 35), malignant fibrous histiocytomas (MFH, 18), fibrosarcomas (FS, 5), and unclassified sarcomas (4). All 19 tumours induced by i.m. injection of Ni 3 S 2 were rhabdomyosarcomas; those induced by s.c. or i.f. injection were mainly MFHs. However, a number of RMSs were also found in groups that received i.a., s.c., and i.f. injections; five FSs also developed in these groups. Four sarcomas induced by s.c. and i.a. injections were not classified. No synovial sarcoma developed.