Premium
Antigenic specificity of human alveolar macrophages and blood monocytes studied by an immunofluorescence technique
Author(s) -
Nakstad Britt,
Lyberg Torstein,
Baklien Kåre,
Boye Nils P.
Publication year - 1989
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711570204
Subject(s) - antigen , peripheral blood mononuclear cell , monoclonal antibody , percoll , immunofluorescence , differential centrifugation , monocyte , antibody , microbiology and biotechnology , lactosylceramide , centrifugation , immunology , chemistry , biology , glycolipid , in vitro , biochemistry
Lung alveolar macrophages (AM) and blood monocytes (BM) are part of the mononuclear phagocyte system (MPS) and have been shown to be composed of several functionally and biochemically distinct subsets. AM and BM were each fractionated into four subfractions by centrifugation over Percoll, 95 per cent of the monocytes having a higher buoyant density than the high‐density AM fraction. Antigen distribution within the cell subfractions was studied by an immunofluorescence technique using a panel of ten monoclonal antibodies (MoAbs) reacting with separate antigenic determinants previously found on mononuclear phagocytes. Several antigens were present on both types of cells (Ki‐M1, Ki‐M6, Leu‐M3, Leu‐M5, 1D5, Dako‐Ma, and HLA‐DR), while other antigens were expressed exclusively on BM (MAS 072 and MAS 081) or on AM (Ki‐M8). Ki‐M1, Leu‐M3, Leu‐M5, and HLA‐DR were expressed to a greater degree on the surface of AM (95–96 per cent of cells) than on BM (5668 per cent), while an inverse relationship was noted for 1D5 antigen (present on 41 per cent of BM and 11 per cent of AM). Ki‐M6 and Dako‐Ma MoAbs recognized an intracellularly restricted antigen present in both AM and BM. Ki‐M1, Ki‐M8, MAS072, MAS081, Leu‐M3, Leu‐M5, 1D5, and HLA‐DR were expressed both in the cytoplasm and at the cell surface. Some antigens (1D5, Leu‐M3, MAS 072, MAS 081, and Dako‐Ma) showed an uneven distribution among subsets of both AM and BM. The variability found in the expression of both membrane and intracellular antigens underlines the remarkable heterogeneity among mononuclear phagocytes and emphasizes the potential value of MoAbs to study the sequential stages of maturation/differentiation within the monocyte/macrophage lineage.