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Immunoelectron microscopic studies of immune complex deposits and basement membrane components in IgA nephropathy
Author(s) -
Woodrow David F.,
Shore Ian,
Moss Jill,
Gower Peter,
Phillips Malcolm
Publication year - 1989
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711570107
Subject(s) - basement membrane , fibronectin , glomerular basement membrane , nephropathy , immunoelectron microscopy , type iv collagen , immune system , immunogold labelling , pathology , extracellular matrix , antibody , chemistry , biology , kidney , laminin , glomerulonephritis , immunology , microbiology and biotechnology , medicine , endocrinology , diabetes mellitus
The relationship between the immune complex deposits of mesangial IgA nephropathy and the basement membrane components, type IV collagen and fibronectin, has been investigated by an indirect immunogold technique in four cases of mesangial IgA disease. Using paraformaldehyde‐fixed, Lowicryl K4M resin‐embedded kidney, IgA, IgM and C3 were localized in the mesangial electron‐dense deposits with 10 and 20 nm gold‐labelled secondary antibodies. In the same glomeruli, type IV collagen and fibronectin were rarely present within the electron‐dense deposits, although both were distributed throughout the remainder of the mesangial matrix with the exception of the subepithelial regions. These two components were also present within the glomerular basement membrane and localized mainly on the endothelial aspect. A similar distribution of the basement membrane components was seen in a control kidney processed in the same way. This technique gives reproducible results and has demonstrated for the first time the relationship between the mesangial immune complex deposits of mesangial IgA nephropathy and the basement membrane components of the matrix in which they are found.