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Immunohistochemical characterization of osteoclasts and osteoclast‐like cells with monoclonal antibody MB1 on paraffin‐embedded tissues
Author(s) -
Chilosi Marco,
Gilioli Eliana,
Lestani Maurizio,
Menestrina Fabio,
FioreDonati Luciano
Publication year - 1988
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711560310
Subject(s) - immunohistochemistry , monoclonal antibody , osteoclast , pathology , chemistry , antibody , microbiology and biotechnology , biology , medicine , immunology , in vitro , biochemistry
In this study we provide evidence that MB1, a newly developed monoclonal antibody which reacts with B lymphocytes and a proportion of T cells and monocytes, can be successfully used for the direct immunohistochemical identification of osteoclasts on paraffin‐embedded surgical specimens. The antigen(s) recognized by MB1 is present at high density in the cytoplasm of osteoclasts of fetal bone and in the multinucleated cells of human giant cell tumour of bone (osteoclastoma), but is weakly expressed or absent in the giant cells of granulomas. MB1 is thus proposed as a new immunohistochemical marker for osteoclasts on paraffin‐embedded material.