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Electron microscopic observations in in situ and microinvasive bronchogenic squamous cell carcinoma
Author(s) -
Imai Tadashi,
Saito Yasuki,
Nagamoto Noriyoshi,
Usuda Katuo,
Takahashi Satomi,
Sagawa Motoyasu,
Sato Masami,
Kanma Keiji,
Suda Shuichi,
Hashimoto Kunihisa,
Nakata Tasuku,
Tagusagawat Kimihiko,
Aso Noboru,
Sato Toshio,
Sato Hirotoshi
Publication year - 1988
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711560309
Subject(s) - cytoplasm , pathology , mitosis , electron microscope , cell , chemistry , intracellular , cell type , basement membrane , biology , microbiology and biotechnology , biophysics , medicine , biochemistry , optics , physics
Seventeen cases of resected in situ and microinvasive bronchogenic squamous cell carcinoma were studied by light and electron microscopy. No definite secretory differentiation was found in any case. Examination of the tumour cells in the basal layer for electron density of cytoplasm, intercellular spaces, and degree of development of cytoplasmic processes showed a variety of cells ranging from type I, where the cytoplasm was dark, development of cytoplasmic processes was good, and the intercellular spaces were large, to type III, where cytoplasmic processes and intercellular spaces were less well developed and the cells were mostly of clear cell type. The tendency to invasion was greater in type III than type I and there was also more marked cellular atypia, more extensive dissolution of basement membrane, a larger number of mitotic figures per 3000 cells in the basal layer, and greater enlargement of nuclear and cytoplasmic areas. A good rank correlation coefficient was obtained. Small dense‐core granules were observed in some cases. These finding suggest the strong possibility that cell kinetics and cellular morphology are related to the development of squamous cell carcinoma.