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Correlation between nor sizes and numbers in non‐Hodgkin's lymphomas
Author(s) -
Crocker John,
Egan Mark J.
Publication year - 1988
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711560308
Subject(s) - nucleolus organizer region , interphase , histology , pathology , nuclear medicine , biology , medicine , nucleus , nucleolus , genetics , microbiology and biotechnology
A series of 36 cases of non‐Hodgkin's lymphomas (NHL) has been studied by means of the argyrophil (AgNOR) method for nuclear organizer regions (NORs). Morphometric analysis of highly magnified photographic images of light microscope preparations of the AgNORs was performed by means of an interactive image analysis system. It was observed that in the low‐grade specimens (where NORs are less frequent than in those of high‐grade histology), the AgNOR sites were highly significantly ( P <0·001) larger than in high‐grade NHL. In the low‐grade lymphomas, the AgNOR maximum diameter ( D max ) ranged from 0·7 to 1·7 μm 2 (mean 1·11μm 2 ) and area ranged from 0·48 to 1·99 μm 2 (mean 1·11 μm 2 ). In contrast, in the high‐grade specimens, D max was from 0·33 to 0·51 μm (mean 0·41μm) and the area ranged from 0·082 to 0·19 μm 2 (mean 0·13 μm 2 ). Thus, a well‐defined inverse relationship was observed between AgNOR numbers and their sizes. There was total separation between low‐ and high‐grade values in this series. This light microscope technique offers some advantages over ultrastructural morphometry of interphase NORs (fibrillar centres).