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Silver‐stained nucleoli and nucleolar organizer region counts are of no prognostic value in thick cutaneous malignant melanoma
Author(s) -
Howat Alec J.,
Giri Dilip D.,
Wright Andrew L.,
Underwood James C. E.
Publication year - 1988
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711560307
Subject(s) - nucleolus organizer region , nucleolus , melanoma , significant difference , silver stain , pathology , black female , nuclear medicine , nucleolar organizer region , medicine , biology , nucleus , cancer research , gender studies , sociology , psychiatry
Nucleolar organizer regions (NORs) can be stained by a simple one‐step silver technique; the black dots formed are termed AgNORs. Often AgNORs are tightly clustered, appearing as one silver‐stained nucleolus (AgNu). We have assessed this technique as a possible prognostic indicator for thick (> 3·0 mm) primary cutaneous malignant melanoma (CMM). Three groups were studied: (A) seven thick CMM that had not metastasized 8–20 years after excision; (B) three thick CMM that developed metastases 6–9 years after excision; and (C) twelve CMM that presented with metastases or developed them within 4 years of excision. Two methods of counting silver‐stained black dots in nuclei were employed: one method counted easily discernible black dots consisting of AgNus and dispersed AgNORs; the other attempted to count actual AgNORs both dispersed and clustered within AgNus. Scores per nucleus by the first method were 1·5–6·7 in group A, 1·1–2·6 in group B, and 1·4–5·4 in group C. AgNOR counts by the second method were 6·2–13·0 in group A, 5·4–8·9 in group B, and 5·3–10·5 in group C. No significant difference was present between groups for scores by either method. Due to the subjectively, technical difficulty, non‐reproducibility, and tedium associated with the second method of attempting to count individuals AgNORs, the first method is recommended. It is concluded that this technique is of no value in predicting prognosis for CMM.