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Tumourigenic transformation of mouse NIH 3T3 cells by transfection with plasmid pSV2neo
Author(s) -
Wallace Jeannie S.,
Fleming Kenneth A.,
Tarin David
Publication year - 1988
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711560114
Subject(s) - transfection , selectable marker , plasmid , transformation (genetics) , biology , neomycin , microbiology and biotechnology , gene , genetics , antibiotics
This study reports high incidence tumourigenic activity of the pSV2neo plasmid demonstrated by transfection of NIH 3T3 cells. The plasmid is frequently used as a dominant selectable marker for confirming successful gene transfection, and the findings indicate a need for caution in interpretation and design of assays for oncogenes which use co‐transfection and subsequent selection with neomycin.