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Lectin binding and desmin staining during bupivicaine‐induced necrosis and regeneration in rat skeletal muscle
Author(s) -
Helliwell Timothy R.
Publication year - 1988
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711550407
Subject(s) - desmin , regeneration (biology) , lectin , skeletal muscle , necrosis , chemistry , staining , pathology , immunohistochemistry , anatomy , microbiology and biotechnology , biology , biochemistry , medicine , vimentin
The processes of bupivicaine‐induced necrosis and regeneration in rat skeletal muscle have been studied with a panel of biotinylated lectins and by immunohistochemical staining for desmin. The results indicate that the binding of lectins to the periphery of muscle fibres is not altered in necrotic and regenerating fibres at the light microscopic level although abnormal cytoplasmic staining by lectins occurs early in necrosis and is also present during regeneration. Desmin staining is lost at an early stage of necrosis and is present at an early stage of regeneration. Desmin staining in combination with morphometrical assessment of the rate of fusion and growth of regenerating fibres provides a powerful method for evaluating the processes of muscle regeneration in experimental situations.