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Optimal preservation of p21 ras immunoreactivity and morphology in paraffin‐embedded tissue
Author(s) -
Going J. J.,
Williams A. R. W.,
Wyllie A. H.,
Anderson T. J.,
Piris J.
Publication year - 1988
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711550302
Subject(s) - paraformaldehyde , immunostaining , staining , pathology , immunohistochemistry , biology , oncogene , in vivo , microbiology and biotechnology , in vitro , chemistry , cell , cell cycle , biochemistry , medicine
Specific immunostaining of p21 ras protein by the well‐characterized pan‐ ros antibody Y13‐259 is achieved in paraffin sections of human and animal tissues fixed in periodate‐lysine‐paraformaldehyde‐dichromate (PLPD). Intensity of staining is as good as in cryostat sections, with superior histological detail. Localization to plasma membrane is demonstrated in rodent cells genetically manipulated to express abundant p21 ras (the FHO5T1 cell line), both in preparations suspended in agar after culture in vitro and in those growing as tumour in vivo . Strong positive staining is observed in neoplasms of human breast and colon, tissues in which there is independent evidence of elevated ras gene expression. The superior morphology afforded by this technique allows clear characterization of p21 ras expression in small premalignant lesions for which other methods of detection of oncogene expression are not appropriate.