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The lectin‐binding characteristics of aflatoxin B 1 induced lesions in the rat liver
Author(s) -
Pritchard Deborah J.,
Butler William H.
Publication year - 1988
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711540310
Subject(s) - basophilic , hepatocellular carcinoma , eosinophilic , pathology , lectin , biology , aflatoxin , immunohistochemistry , microbiology and biotechnology , cancer research , medicine , food science
Hepatocellular carcinoma was induced in rats by administering aflatoxin B 1 (AFB 1 ) for 6 weeks. Malignant tumours were preceded by foci and nodules of altered hepatocytes of three histological types, composed of basophilic, eosinophilic, and vacuolated cells. In addition, there were areas of altered hepatocytes that were considered as hyperplastic. Lectins were used as histochemical markers to compare the expression of membrane glycoproteins in hepatocellular carcinomas and hepatic nodules with non‐nodular or control hepatocytes. There were marked changes in the lectin‐binding patterns of the hepatocellular carcinoma cells and the eosinophilic nodules. The lectin‐binding patterns of basophilic nodules, vacuolated nodules, and hyperplastic areas were similar to non‐nodular or untreated hepatocytes. The similarity in the lectin‐binding changes of the eosinophilic nodules and hepatocellular carcinomas suggests that the eosinophilic nodules may be an early stage in the development of carcinoma.