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Leu‐M1 antigen expression in acute leukaemias
Author(s) -
De Mascarel Antoine,
Coindre Jean Michel,
De Mascarel Isabelle,
David Bernard,
Trojani Monique,
Broustet Antoine
Publication year - 1987
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711530306
Subject(s) - null cell , immunoperoxidase , lymphoblast , lymphoblastic lymphoma , lymphoma , myeloid , antibody , antigen , b cell , medicine , t cell , pathology , immunology , biology , cell culture , monoclonal antibody , immune system , genetics
Leu‐M1 is a differentiation antigen present in human myelomonocytic cells. Seventy‐seven acute leukaemias were retrospectively stained with anti‐Leu‐M1 using the immunoperoxidase technique on Bouin‐fixed paraffin‐embedded sections. The subjects were 44 acute lymphoblastic leukaemias (ALL) and 33 acute myeloid leukaemias (AML) previously characterized by cytochemical and immunologic (cell suspension) methods. Leu‐M1 was positive in all the AML and in half of the ALL cases. These results suggest that Leu‐M1 does not allow differentiation between AML and ALL. For the ALL cases Leu‐M1 was positive in 15/28 B‐cell types, 4/12 T‐cell type and 3/4 ‘null’‐cell type cases. Thus, this antibody is of no assistance in defining types B, T, or ‘null’ in ALL. Leu‐M1 was also studied on paraffin sections of 34 high grade malignant lymphomas. The antibody was negative in all 13 B‐cell lymphomas (lymphoblastic: 6; immunoblastic: 7) and in all 4 ‘null’ cell lymphomas. It was positive in 4/9 peripheral T‐cell type, the other T‐cell lymphomas (lymphoblastic: 5; immunoblastic:3) remaining negative. Thus, Leu‐M1 may be positive in T‐cell lymphomas but it is negative in B‐cell lymphomas and is always negative in B or T lymphoblastic types. It seems that lymphoblasts are Leu‐M1 negative in non‐Hodgkin's lymphoma and may be Leu‐M1 positive in leukaemias.