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Electron microscopic identification of aberrant melanosomes using a combined DOPA/Warthin–Starry technique
Author(s) -
Rennison Alexander,
Duff Carol,
McPhie James L
Publication year - 1987
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711520412
Subject(s) - melanosome , identification (biology) , pathology , anatomy , chemistry , medicine , biology , melanin , biochemistry , botany
A method combining the DOPA and Warthin–Starry techniques is described in order to positively establish the nature of pleomorphic granules observed in the cytoplasm of cells of putative amelanotic melanoma. The technique identifies these granules as aberrant melanosomes by discretely depositing electron dense silver on suitably prepared sections of DOPA‐treated tissue blocks.