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Organ culture of human gastric mucosa and gastric cancers. 1. Morphological aspects
Author(s) -
Wilson Nigel W.,
Macartney James C.
Publication year - 1986
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711500207
Subject(s) - organ culture , gastric mucosa , intestinal metaplasia , in vivo , foveolar cell , pathology , biology , cancer , epithelium , in vitro , thymidine , stomach , metaplasia , enterochromaffin like cell , medicine , biochemistry , genetics , microbiology and biotechnology
Abstract An organ culture system for explants of human gastric mucosa and gastric cancer from surgical resection specimens is described. Pyloric mucosa has been successfully maintained in vitro for up to 120 h, and gastric carcinoma for up to 48 h. Morphological preservation by light and electron microscopy was good, although some simplification of the glandular pattern occurred in normal mucosa with longer periods of in vitro culture. [ 3 H]‐thymidine and [ 3 H]‐leucine uptake was assessed by autoradiography, and patterns of uptake resembled those described in vivo in normal gastric mucosa and gastric intestinal metaplasia. Labelling indices with [ 3 H]‐thymidine were however low compared with in vivo estimates. Successful organ culture of human gastric tissue offers a useful method for investigating factors affecting growth control in normal and neoplastic gastric epithelium.