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Cluster differentiation antigen expression, proliferative activity and clinical stage in centroblastic centrocytic lymphomas
Author(s) -
Williamson J. M. S.,
Grigor I.,
Smith M. E. F.,
Holgate C. S.,
Quirke P.,
Bird C. C.,
Alison D. L.,
Child J. A.
Publication year - 1986
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711500109
Subject(s) - stage (stratigraphy) , antigen , cluster (spacecraft) , expression (computer science) , biology , lymphoma , cluster of differentiation , cancer research , microbiology and biotechnology , pathology , immunology , medicine , cell , genetics , computer science , paleontology , programming language
Using a panel of B‐cell antibodies recognizing clusters of leucocyte differentiation antigens, immunostaining patterns of eight reactive lymph nodes and 28 centroblastic/centrocytic and centrocytic lymphomas have been studied. Centroblastic/centrocytic and centrocytic lymphomas retained many of the B‐cell differentiation antigens and neoplastic follicles partially recapitulated the staining patterns observed in reactive follicles. Centrocytic lymphomas usually expressed a heavy chain mantle zone‐like phenotype. Nearly one‐half of follicular lymphomas showed extension of neoplastic cells into interfollicular areas as evidenced by positivity for CD 10 (common acute lymphoblastic leukaemia) and/or CD9 (immature B‐cell) and CD23 (B‐blast cell) antigens. Cases showing interfollicular involvement also manifested considerable phenotypic heterogeneity. Light chain restriction could not be used to determine interfollicular involvement because of the presence of many non‐neoplastic cells. Most follicular lymphomas retained a polyclonal mantle around at least some neoplastic follicles and in no case was a monoclonal mantle seen. Most lymphomas (16/21) were diploid when examined by flow cytometry. Diploid tumours exhibiting interfollicular lymphomatous involvement had high proliferation (S + G 2 ) fractions and these lymph nodes were usually derived from patients with widespread disease. Tumours containing a high percentage of cells in the G 0 /G 1 phases displayed fewer B‐cell differentiation antigens than tumours with low G 0 /G 1 fractions.