Premium
Expression of epithelial and neural antigens in small cell and non small cell lung carcinoma
Author(s) -
Moss Fiona,
Bobrow Lynda G.,
Sheppard Mary N.,
Griffiths Meryl,
Rowe Deborah,
Beverley Peter C. L.,
Addis Bruce,
Souhami Robert L.
Publication year - 1986
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711490205
Subject(s) - immunoperoxidase , monoclonal antibody , antigen , pathology , immunohistochemistry , antibody , small cell carcinoma , adenocarcinoma , carcinoma , biology , cell , small cell lung carcinoma , large cell , staining , stain , cancer research , cancer , medicine , immunology , genetics
Seventy‐one lung carcinomas from 66 different patients were stained with a panel of monoclonal antibodies. Twenty‐nine were small cell lung carcinoma (SCLC), 15 adenocarcinomas, 17 squamous carcinomas and 10 large cell carcinomas. Three of the monoclonal antibodies recognize different cytokeratins, three recognize other epithelial antigens and one recognizes a neural antigen. Both formalin‐fixed and cryopreserved tumours were studied using an indirect immunoperoxidase method. 23/29 SCLC reacted with all but one of the antibodies which recognize epithelial antigens. This staining was similar to that seen in non small cell lung carcinomas (NSCLC) and provides further evidence that SCLC are true epithelial tumours. All but one of the SCLC stained with the antibody recognizing a neural antigen. This antibody did not stain squamous or adenocarcinomas. However, four of the the large cell carcinomas stained well with this antibody, suggesting that SCLC and some large cell carcinomas share a common pathway of differentiation. There were variations of staining seen both within and between tumours. This has obvious implications if immunotargetting with monoclonal antibodies is to be used diagnostically or therapeutically.