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Electron microscopic localization of cell‐surface markers in tissue sections using monoclonal and gold‐conjugated antibodies
Author(s) -
Rooney N.,
Day C.,
Gray T.,
Underwood J. C. E.
Publication year - 1986
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711480106
Subject(s) - monoclonal antibody , antibody , antigen , ultrastructure , chemistry , conjugated system , cell , microbiology and biotechnology , biophysics , biology , immunology , anatomy , biochemistry , organic chemistry , polymer
We describe a method for the ultrastructural localization of surface markers on cells in intact tissue sections. Using a primary monoclonal antibody to a surface marker followed by a second stage gold‐labelled antibody, surface antigens can be specifically localized. This enables cellular interactions to be studied in solid tissues.