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A convenient method for in situ processing of cultured cells for cytochemical localization by electron microscopy
Author(s) -
Ballou Rick J.,
Simpson William G.,
Tseng Michael T.
Publication year - 1985
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711470310
Subject(s) - electron microscope , microscopy , transmission electron microscopy , in situ , sample preparation , sample (material) , scanning electron microscope , materials science , biomedical engineering , nanotechnology , computer science , biological system , biophysics , chemistry , pathology , biology , chromatography , optics , composite material , medicine , physics , organic chemistry
The effects of various exogenous agents on subcellular structures is of importance to many investigators and can be critically evaluated by the use of cytochemical techniques and transmission electron microscopy. Therefore various cell culture techniques have become increasingly important in biological research in order to help determine these effects. Presently, most existing methods for processing anchorage‐dependent cultures for electron microscopy utilize cells grown on either glass or plastic substrates. Therefore various coating substances have been applied to the culture surfaces to facilitate removal of the sample, however, incomplete separation and sample fracture often results. Here we present a simple method of in situ processing of samples for electron microscopy involving the use of detachable chamber slides. This method allows for the use of a quick processing procedure that results in a complete separation of the sample from the glass slide.