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HLA‐DR expression in a human colonic carcinoma cell linexs
Author(s) -
Lampert Irvin A.,
Kirkland Susan,
Farrell Siobhan,
Borysiewicz Leszek K.
Publication year - 1985
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711460407
Subject(s) - adenocarcinoma , human leukocyte antigen , pathology , cell culture , carcinoma , cell , colonic carcinoma , recombinant dna , staining , biology , cancer research , antigen , immunology , medicine , cancer , biochemistry , genetics , gene
A human colonic carcinoma cell line (HCA‐7) isolated from a well differentiated mucoid adenocarcinoma of the colon has been maintained in vitro for 3 years. It spontaneously synthesizes HLA‐DR which is mainly intracytoplasmic. Stimulation with lymphocyte conditioned medium and recombinant γ‐interferon results in enhanced synthesis of HLA‐DR and the appearance of the antigen on the cell surface. A dose response study showed that maximal stimulation of the culture was achieved with 50 units/ml of recombinant γ‐interferon. Staining for HLA‐DR was uneven being confined to focal areas of the monolayer, which is similar to the focal expression of HLA‐DR seen in sections of adenocarcinoma of the colon. The functional significance of this phenomenon is unclear, but it may explain the presence of lymphoid infiltrates in tumours.