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Regulatory peptides in Barrett's oesophagus
Author(s) -
Buchan Alison M. J.,
Grant Susan,
Freeman H. J.
Publication year - 1985
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711460310
Subject(s) - argentaffin , gastrin , somatostatin , motilin , staining , enteroendocrine cell , epithelium , medicine , pathology , biology , chemistry , secretion , endocrine system , hormone
Barrett's epithelium refers to the presence of ectopic mucosal types in the squamous‐lined oesophagus. Previous studies have documented argentaffin and argyrophil‐positive cells as well as gastrin‐like immunoreactivity in oesopha‐geal tissue extracts from patients with Barrett's mucosa. In the present study, 125 oesophageal biopsies obtained under direct vision at endoscopy from 22 patients with Barrett's oesophagus were systematically studied using fluorescence and peroxidase antiperoxidase single and double‐staining immunocytochemical methods employing highly specific antibodies to localize the following peptide‐containing cell types in Barrett's mucosa: gastrin, somatostatin, gastric inhibitory polypeptide, motilin, neurotensin and pancreatic glucagon. In addition, EC cells were localized using a cytochemical silver staining method. The results of this study indicate that EC cells and gastrin‐ and somatostatin‐containing endocrine cells are detectable in Barrett's epithelium.