Premium
Identification of macrophages and smooth muscle cells in human atherosclerosis using monoclonal antibodies
Author(s) -
Aqel N. M.,
Ball R. Y.,
Waldmann H.,
Mitchinson M. J.
Publication year - 1985
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711460306
Subject(s) - immunoperoxidase , monoclonal antibody , pathology , macrophage , cytoplasm , monoclonal , antibody , smooth muscle , biology , chemistry , medicine , microbiology and biotechnology , immunology , in vitro , biochemistry , endocrinology
Sections of human atherosclerotic plaques were stained by the indirect immunoperoxidase technique using three rat anti‐human monoclonal antibodies: YAML 501·4 anti‐leukocyte‐common' (T200) antigen; YTH 8·18 (anti‐macrophage cytoplasm); and YPC 1/3·12 (anti‐smooth muscle cell). The cells of diffuse intimal thickening were almost all smooth muscle cells but there were rare subendothelial macrophages. Focal lesions, in contrast, contained numerous macrophages as well as smooth muscle cells. Macrophage ‘foam cells’ were particularly numerous in fatty streaks and in advanced fibro‐fatty plaques, but were less conspicuous in focal fibro‐elastic lesions. The results confirm that macrophages are an important component of atherosclerotic plaques and suggest that they may have a significant role in atherogenesis in man.