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Immunolocalization of prealbumin: Distribution in normal human tissue
Author(s) -
Liddle Christine N.,
Reid W. A.,
Kennedy J. S.,
Miller I. D.,
Horne C. H. W.
Publication year - 1985
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/path.1711460205
Subject(s) - immunoperoxidase , staining , glucagon , transthyretin , gastrointestinal tract , biology , somatostatin , immunohistochemistry , distribution (mathematics) , enteroendocrine cell , islet , medicine , endocrinology , antibody , chemistry , insulin , monoclonal antibody , biochemistry , endocrine system , hormone , immunology , mathematical analysis , mathematics , genetics
Using an indirect immunoperoxidase staining technique we have demonstrated the plasma protein, prealbumin, in the cells of the pancreatic islets of Langerhans. Serial sections stained with antisera to glucagon, insulin and somatostatin revealed that the distribution of the prealbumin stained cells matched that of the glucagon A‐cells. Absorption and cross‐absorption experiments with prealbumin and glucagon showed that the staining was specific. We also found positive staining for prealbumin in single cells in the mucosa of the gastrointestinal tract, mainly stomach and colon, and kidney tubules.